Expression of vascular endothelial growth factor related to 72-kilodalton metalloproteinase immunostaining in patients with serous ovarian tumors

✍ Scribed by G. Gioele Garzetti; Andrea Ciavattini; Guendalina Lucarini; Armanda Pugnaloni; Michele De Nictolis; Silvana Amati; Carlo Romanini; Graziella Biagini

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 90 KB
• Volume: 85
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/(sici)1097-0142(19990515)85:10<2219::aid-cncr17>3.0.co;2-s

✦ Synopsis

The aim of the current study was to investigate the expression of vascular endothelial growth factor (VEGF) by neoplastic cells in patients with serous ovarian tumors. The correlation between neoangiogenesis and 72-kilodalton metalloproteinase (MMP2) immunostaining also was evaluated.

METHODS.

Fifty-eight patients with serous ovarian tumors who were treated at the Institute of Gynecology and Obstetrics of Ancona University (Ancona, Italy) were used as the study population; 10 women had serous cystoadenoma, 16 women had a serous borderline tumor, and 32 women had invasive cystoadenocarcinoma.

Expression of VEGF and MMP2 was evaluated immunohistochemically by polyclonal antibody anti-VEGF (dilution, 1:100) and affinity purified, rabbit anti-MMP2, formalin fixed, paraffin embedded tissue. Positive staining was expressed as a percentage of positive cells per 10 3 counted neoplastic cells.