## Abstract Lactate dehydrogenase (LDH) isozyme 1 (__B__~4~), 5 (__A__~4~), and βXβ (Testis or Sperm type) have been partially purified from mouse tissues. The following studies were carried out on the three isozymes: K~m~ and optimum substrate concentration for pyruvate, Ξ±βoxoβbutyrate, Ξ±βoxoβval
Expression of lactate dehydrogenase isozyme X in haploid cells of pigeon testis
β Scribed by Jose Moreno; Cristina N. Gardenal; Agustin Aoki; Antonio Blanco
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 380 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0300-8177
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Starch-gel electrophoresis revealed that the C4 isozyme of lactate dehydrogenase (LDHC4) predominated in the gelatinous, postcranial body mass of metamorphosing leptocephalous larvae of the bonefish (Albula sp.), a primitive teleost fish. At the end of metamorphosis, when the juvenile stage was reac
## Abstract An unusual pattern of LDH isozymes was observed by gel electrophoresis of an extract of a human testis. This isozyme composition is consistent with an allelic variant of Ldhβc.
Extensive extraction studies with Triton X-100 revealed only LDH-1 (B4) but no trace of LDH-5 (A4) in one-cell and two-cell mouse and rat embryos. The LDH isozyme pattern of preimplantation mouse embryos changes from the maternally inherited B subunit isozyme (LDH-1) to a pattern dominated by LDH-5
Early studies, using kinetic methods, suggested that the isozyme pattern of lactate dehydrogenase in various cells oscillated with time. More recent electrophoretic studies on murine erythroleukaemic cells (which exhibit only one isozyme) indicated very high frequency variations (period 2min or less