Expression of Intracellular Hemoglobin Improves Protein Synthesis in Oxygen-Limited Escherichia coli
β Scribed by Khosla, Chaitan ;Curtis, Joseph E. ;DeModena, John ;Rinas, Ursula ;Bailey, James E.
- Book ID
- 109907683
- Publisher
- Nature Publishing Group
- Year
- 1990
- Tongue
- English
- Weight
- 663 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1087-0156
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In a rifS/rifR heterodiploid strain of E. coli, a 4 minute pulse of rifampicin can induce a prolonged (greater than 60 min) increase in the rate of synthesis of the RNA polymerase subunits, beta and beta'. The application of a constraint on the fidelity of protein synthesis during, but not after, th
## Abstract This article reports the cellβfree expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cellβfree synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led
The rate of synthesis of total cellular proteins has been studied by pulse labelling cells at various periods after irradiation with UV or gamma-rays, after treatment with mitomycin C (MMC) or after expression of the temperature sensitive mutation tif. Subsequent gel electrophoresis and autoradiogra