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Expression of herpes simplex virus type 1 recombinant thymidine kinase and its application to a rapid antiviral sensitivity assay

✍ Scribed by Tomoyuki Shiota; Wang Lixin; Mutsuyo Takayama-Ito; Itoe Iizuka; Momoko Ogata; Masanori Tsuji; Hidekazu Nishimura; Shuichi Taniguchi; Shigeru Morikawa; Ichiro Kurane; Masashi Mizuguchi; Masayuki Saijo


Book ID
113458697
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
629 KB
Volume
91
Category
Article
ISSN
0166-3542

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An optimized thymidylate kinase assay, b
✍ Anders R. KarlstrΓΆm; J.Simon Gronowitz πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 1020 KB

The biological synthesis and purification of 5-[125I]iododeoxyuridine monophosphate (IdUMP) are described. The specificity of IdUMP as substrate in the thymidylate monophosphate kinase (TMPK) assay is demonstrated, and a 100-fold gain in sensitivity as compared to the conventional TMPK assay is show