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Expression of functional PDGF β receptors in a human large-cell lung-carcinoma cell line

✍ Scribed by K. Forsberg; J. Berch; B. Westermark

Publisher
John Wiley and Sons
Year
1993
Tongue
French
Weight
612 KB
Volume
53
Category
Article
ISSN
0020-7136

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✦ Synopsis

In this study we have investigated a panel of lung-cancer cell lines, both of small-cell carcinoma and non-small-cell type, for the expression of receptors for platelet-derived growth factor. Although we found mRNA expression for the P-type receptor on one small-cell and one non-small-cell line and a-type receptor mRNA expression on one small-cell-cancer cell line, only the p receptors of the non-small-cell line (H-157) proved to be functional. Thus, the cell line H-I57 displayed specific binding of 1251-PDGF-BB in addition to mRNA expression of the 6-kb transcript for the PDGF f3 type receptor. Further evidence for the presence of functional PDGF P receptors in H-I57 cells was obtained from an in vitro kinase assay, which demonstrated a ligand-induced receptor autophosphorylation as well as the phosphorylation of a number of potential substrates associated with the activated receptor.

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