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Expression of Epstein-Barr virus EBNA1 protein in Escherichia coli: Purification under nondenaturing conditions and use in DNA-binding studies

✍ Scribed by Naïma Bouallag; Claire Gaillard; Vincent Maréchal; François Strauss

Book ID: 116828993
Publisher: Elsevier Science
Year: 2009
Tongue: English
Weight: 408 KB
Volume: 67
Category: Article
ISSN: 1046-5928
DOI: 10.1016/j.pep.2009.04.009

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## Abstract Epstein‐Barr virus (EBV) encoded DNA polymerase (POL) was cloned and over‐expressed in __Escherichia coli__. Western blot analysis confirmed the presence of antibody to this POL protein in sera from nasopharyngeal carcinoma (NPC) patients. By Western blot analysis, moderate to high conc