Expression of Epstein-Barr-virus-associated membrane antigen in Raji cells superinfected with two different virus strains
✍ Scribed by Gottfried Dölken; George Klein
- Book ID
- 119009253
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 366 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0042-6822
No coin nor oath required. For personal study only.
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## Abstract In an attempt to qualitatively identify the membrane antigen (MA) complex induced by Epstein‐Barr virus (EBV) infection of lymphoblastoid cells, superinfected Raji cells were surface labelled with ^125^I by the lactoperoxidase method and solubilized with Triton X‐100, then the ^125^(‐la
In the presence of canavanine and the absence of arginine, superinfected Raji cells synthesize a limited spectrum of proteins (primary proteins). After the removal of canavanine at 8 h post infection, the cells proceed after a lag phase of 2-3 h to synthesize a second group of proteins. The appearan
## Abstract A double antibody sandwich ELISA has been established for the detection and quantitation of EBV‐associated early antigens (EA) in IUdR‐induced Raji cells. The EA complex extracted from Raji cells could be separated by ion exchange chromatography and isoelectric focusing into several com