Expression of enzymatically active enkephalinase (neutral endopeptidase) in mammalian cells

✍ Scribed by Cornelia M. Gorman; David Gies; Peter R. Schofield; Helen Kado-Fong; Bernard Malfroy

• Book ID: 102876222
• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 539 KB
• Volume: 39
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240390307

✦ Synopsis

A cDNA encoding the rat enkephalinase protein (neutral endopeptidase; EC 3.4.24.11) has been constructed from overlapping XgtlO cDNA clones. This cDNA was inserted into an expression plasmid containing the cytomegalovirus enhancer and promoter. When transfected with this plasmid, Cos 7 cells transiently expressed the enkephalinase protein in a membrane-bound state. Recombinant enkephalinase recovered in solubilized extracts from transfected Cos 7 cells was enzymatically active and displayed properties similar to those of the native enzyme with respect to sensitivity to classical enkephalinase inhibitors.