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Expression of deletion mutants of the hepatitis B virus protein HBx in E. coli and characterization of their RNA binding activities

✍ Scribed by Edmilson Rui; Patrı́cia Ribeiro de Moura; Jörg Kobarg

Book ID
117365632
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
659 KB
Volume
74
Category
Article
ISSN
0168-1702

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The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is considered to possess RNA-dependent RNA polymerase (RdRp) activity and to play an essential role for the viral replication. In this study, we expressed the NS5B protein of 65 kd by a recombinant baculovirus. With the highly purified N