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EXPRESSION OF CLONED CHOLERA ENTEROTOXIN GENE IN ESCHERICHIA COLI AND POSSIBILITIES OF VACCINE DEVELOPMENT

โœ Scribed by Gennaro, MariaL; Greenaway, PeterJ; Broadbent, DavidA

Book ID
121929974
Publisher
The Lancet
Year
1982
Tongue
English
Weight
677 KB
Volume
319
Category
Article
ISSN
0140-6736

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โœ Paul, Kalidas ;Ghosh, Subrata K. ;Das, Jyotirmoy ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Springer ๐ŸŒ English โš– 645 KB

A library containing more than 80% of the Vibrio cholerae genome was constructed by cloning BamH1 restriction fragments into pBR322. Using interspecific complementation of an Escherichia coli recA mutant with plasmids containing the gene bank of V. cholerae, a recA-like gene was identified. The reco