Expression of chloramphenicol acetyltransferase in Bacillus subtilis under the control of a phytoplasma promoter
β Scribed by S. Palmano; B.C. Kirkpatrick; G. Firrao
- Book ID
- 117516887
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 183 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Two non-homologous chloramphenicol (Cm) acetyltransferase (CAT) genes, designated catA and catB, were cloned from Clostridium butyricum type strains and characterized by restriction mapping. Both genes are efficiently expressed in Escherichia coli and Bacillus subtilis. In contrast to analogous gene
The Escherichia coli lacUV5 promoter is used inefficiently by the major vegetative form of Bacillus subtilis RNA polymerase, despite very close adherence in the -35 and -10 regions to consensus sequences for promoters recognized by this enzyme. To select derivatives of this promoter with increased a