Two non-homologous chloramphenicol (Cm) acetyltransferase (CAT) genes, designated catA and catB, were cloned from Clostridium butyricum type strains and characterized by restriction mapping. Both genes are efficiently expressed in Escherichia coli and Bacillus subtilis. In contrast to analogous gene
Expression of chloramphenicol acetyltransferase in Bacillus subtilis under the control of a phytoplasma promoter
β Scribed by S. Palmano; B.C. Kirkpatrick; G. Firrao
- Book ID
- 109324968
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 182 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The Escherichia coli lacUV5 promoter is used inefficiently by the major vegetative form of Bacillus subtilis RNA polymerase, despite very close adherence in the -35 and -10 regions to consensus sequences for promoters recognized by this enzyme. To select derivatives of this promoter with increased a
High levels of poly(A) + RNAs homologous t o certain endogenous retrovirus-related DNA sequences are frequently seen in carcinogen-transformed rodent cells. To explore the underlying mechanism, transient expression assays were done to determine whether carcinogen-or radiation-transformed C3H lOTl I2
The recA gene of Proteus mirubilk (recApm) has been cloned into the Pat1 site of the Bacillus promoter-probe plasmid pPL603. When present on this plasmid, the rectlpm') gene is expressed in B. subtilie under the control of its own transcriptional and translational signals. It is concluded that the h