Expression of canine parvovirus-β-galactosidase fusion proteins in Escherichia coli
✍ Scribed by Smith, Susan; Halling, Shirley M.
- Book ID
- 122769352
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 838 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0378-1119
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Covalently immobilized biotin was used as a biospecific adsorbant to investigate the application of streptavidin as an affinity domain for simultaneous purification and immobilization of recombinant proteins. A streptavidinp-galactosidase fusion protein was constructed and tested as a model system.
The tolerance of the -galactosidase C-terminus to foreign protein fusions has been explored by using different-sized derivatives of the chimeric protein LACVP1. While the molecular mass of the partner domain shows a minor influence on protein toxicity for the producing E. coli cells, it dramaticall