## Abstract Recombinant adenoviral vectors have been shown to be potential new tools for a variety of musculoskeletal defects. Much emphasis in the field of orthopedic research has been placed on developing systems for the production of bone. This study aims to determine the necessary conditions fo
Expression of BMP-2 by Rat Bone Marrow Stromal Cells in Culture
β Scribed by L. X. Bi; D. J. Simmons; E. Mainous
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 608 KB
- Volume
- 64
- Category
- Article
- ISSN
- 1432-0827
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To determine how low intensity pulsed ultrasound alters gene expression in rat bone marrow stromal cells and to see if combining this stimulation with BMP-2, cells were pre-cultured for eight days in the presence of 50 microg/ml ascorbic acid and then exposed to either low intensity US or 100 ng/ml
## Abstract Rat bone marrow stromal cells were cultured on either Ra (0.14 ΞΌm) or Ra (5.8 ΞΌm) Ti~6~Al~4~V discs for 24 or 48 h. Cells on the Ra (0.14 ΞΌm) surface showed typical fibroblastic morphology, whereas cells on the Ra (5.8 ΞΌm) surface were in clusters with a more epithelial appearance. RNA