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Expression of atrial natriuretic factor as a cleavable fusion protein with chloramphenicol acetyltransferase in Escherichia coli

✍ Scribed by Colin W. DYKES; A. Brett BOOKLESS; Barry A. COOMBER; Stewart A. NOBLE; David C. HUMBER; Adrian N. HOBDEN

Book ID: 115125810
Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 849 KB
Volume: 174
Category: Article
ISSN: 1432-1327
DOI: 10.1111/j.1432-1033.1988.tb14113.x

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We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,