## Abstract The influence of linker design on fusion protein production and performance was evaluated when a family 9 carbohydrateβbinding module (CBM9) serves as the affinity tag for recombinant proteins expressed in __Escherichia coli__. Two bioinformatic strategies for linker design were applied
β¦ LIBER β¦
A method for the amidation of recombinant peptides expressed as intein fusion proteins in Escherichia coli
β Scribed by Cottingham, Ian R.; Millar, Alan; Emslie, Elisabeth; Colman, Alan; Schnieke, Angelika E.; McKee, Colin
- Book ID
- 109908014
- Publisher
- Nature Publishing Group
- Year
- 2001
- Tongue
- English
- Weight
- 207 KB
- Volume
- 19
- Category
- Article
- ISSN
- 1087-0156
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We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,