Expression of a synthetic protein-based polymer (elastomer) gene inAspergillus nidulans
โ Scribed by R. W. Herzog; N. K. Singh; D. W. Urry; H. Daniell
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 319 KB
- Volume
- 47
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
We report here expression of a protein based polymer gene (Gly-Val-Gly-Val-Pro)m , coding for three amino acids in a pentamer sequence repeated 121 times via the nuclear' ge~ome of tobacco cells. Transformed tobacco cells were obtained by particle bombardment. Stably transformed cells show the prese
The Escherichia coli aspartase gene aspA has been expressed in the fungus Aspergillus nidulans using the powerful constitutive gpdA promoter and trpC terminator, both from A. nidulans. Multiple, but not single, copies of aspA overcome nutritional deficiencies resulting from the loss of catabolic NAD
A homologous transformation system for Aspergillus oryzae is described. The system is based on an A. owzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrG) and the vector pAO4-2, which contains a functional A. oryzae pyrG gene as selection marker. Transformation of the A. owzae pyrG m