✦ LIBER ✦

Nuclear expression of an environmentally friendly synthetic protein based polymer gene in tobacco cells

✍ Scribed by X. Zhang; C. Guda; R. Datta; R. Dute; D. W. Urry; H. Daniell

Publisher: Springer Netherlands
Year: 1995
Tongue: English
Weight: 427 KB
Volume: 17
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00189210

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✦ Synopsis

We report here expression of a protein based polymer gene (Gly-Val-Gly-Val-Pro)m , coding for three amino acids in a pentamer sequence repeated 121 times via the nuclear' ge~ome of tobacco cells. Transformed tobacco cells were obtained by particle bombardment. Stably transformed cells show the presence of the polymer gene in the tobacco nuclear genome (2-5 copies); introduced polymer gene is transcribed efficiently as revealed by Northern blots; Western blots show the presence of the polymer protein. To the best of our knowledge, this report represents the first demonstration of expression of a synthetic gene (with no natural analog) in higher plants.

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