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Expression, Lipoylation and Structure Determination of Recombinant Pea H-Protein in Escherichia coli

โœ Scribed by David Macherel; Jacques Bourguignon; Eric Forest; Magali Faure; Claudine Cohen-Addad; Roland Douce

Book ID
115131732
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
958 KB
Volume
236
Category
Article
ISSN
1432-1327

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Elevated Fis expression enhances recombi
Elevated Fis expression enhances recombinant protein production in Escherichia coli
โœ Richard Richins; Tin Htay; Pauli Kallio; Wilfred Chen ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 134 KB ๐Ÿ‘ 2 views

A genetic strategy to enhance recombinant protein production is discussed. A small DNA bending protein, Fis, which has been shown to activate rRNA synthesis upon a nutrient upshift, was overexpressed in E. coli strain W3110 carrying vector pUCR1. Overexpres- sion of Fis during exponential growth was