Glutathione is excreted in a dose-dependent, non-stoichiometric fashion from Saccharomyces cerevisiae cells expressing and secreting Bovine Pancreatic Trypsin Inhibitor (BPTI), a small, disulfide-bonded protein. Glutathione excretion commences 40 hours following induction of BPTI synthesis. Expressi
Expression Level Tuning for Optimal Heterologous Protein Secretion in Saccharomyces cerevisiae
β Scribed by Rajesh N. Parekh; K. Dane Wittrup
- Book ID
- 109387948
- Publisher
- American Institute of Chemical Engineers
- Year
- 1997
- Tongue
- English
- Weight
- 122 KB
- Volume
- 13
- Category
- Article
- ISSN
- 8756-7938
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## Abstract In this study, secretory processing of cellβsurface displayed Aga2p fusions to bovine pancreatic trypsin inhibitor (BPTI) and the single chain Fv (scFv) antibody fragment D1.3 are examined. BPTI is more efficiently processed than D1.3 both when secreted and surfaceβdisplayed, and D1.3 e
The two most commonly used amino acid media supplements do not improve the specific rate of secretion of Schizosuccharomycespombe acid phosphatase in S. cerevisiae relative to unsupplemented Synthetic Dextrose (SD) media. New amino acid supplement formulations have been obtained which produce an B-f