Optimization of amino acid supplements for heterologous protein secretion inSaccharomyces cerevisiae

✍ Scribed by K. D. Wittrup; V. Benig

• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 540 KB
• Volume: 8
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00161582

✦ Synopsis

The two most commonly used amino acid media supplements do not improve the specific rate of secretion of Schizosuccharomycespombe acid phosphatase in S. cerevisiae relative to unsupplemented Synthetic Dextrose (SD) media. New amino acid supplement formulations have been obtained which produce an B-fold increase in total phosphatase secretion, and a 70% increase in specific phosphatase secretion.