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Expression-independent consumption of substrates in cell-free expression system from Escherichia coli

✍ Scribed by R.G. Kim; C.Y. Choi

Book ID
118060071
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
159 KB
Volume
84
Category
Article
ISSN
0168-1656

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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led