Expression and shedding of intercellular adhesion molecule 1 and lymphocyte function–associated antigen 3 by normal and scleroderma fibroblasts.

✍ Scribed by Xu Shi-Wen; Manvinder Panesar; Rama Vancheeswaran; Justin Mason; Dorian Haskard; Carol Black; Irwin Olsen; David Abraham

• Book ID: 102750675
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 895 KB
• Volume: 37
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780371119

✦ Synopsis

Objective. To examine intercellular adhesion molecule 1 (ICAM-1) and lymphocyte functionassociated antigen 3 (LFA-3) in cultures of normal and systemic sclerosis (SSc) dermal fibroblasts.

Methods. The surface and soluble forms of ICAM-1 and LFA-3 were measured by flow cytometry and capture enzyme-linked immunosorbent assay, respectively.

Results. Surface ICAM-1 was significantly higher on SSc fibroblasts compared with normal controls. P-estradiol did not directly enhance ICAM-1 or LFA-3 expression in either normal or SSc cells, but significantly augmented the cytokine-induced increase in ICAM-1. Soluble ICAM-1 (SICAM-1) and sLFA-3 were detected in fibroblast cultures. While no difference was found in the level of sLFA-3, the shedding of SICAM-1 was significantly increased (P < 0.001) in cells from SSc patients.