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Expression and Purification of Imidazole Glycerol Phosphate Synthase from Saccharomyces cerevisiae

✍ Scribed by Sridar V. Chittur; Yuan Chen; V.Jo Davisson

Book ID
115645625
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
148 KB
Volume
18
Category
Article
ISSN
1046-5928

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An NAD-dependent glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate:NAD+ oxidoreductase, EC 1.1.1.8) has been isolated and purified from Saccharomyces cerevisiae by affinity and exclusion chromatography. The enzyme was purified 5 1 OO-fold to a specific activity of 158. It has a molecular w