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Fast purification and kinetic studies of the glycerol-3-phosphate dehydrogenase from the yeast Saccharomyces cerevisiae

✍ Scribed by Jingmin Cai; Markus Pietzsch; Uwe Theobald; Manfred Rizzi


Book ID
119132399
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
783 KB
Volume
49
Category
Article
ISSN
0168-1656

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Purification and some properties of sn-g
✍ Joseph R. Merkel; Martin Straume; Susan A. Sajer; Robert L. Hopfer πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 810 KB

An NAD-dependent glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate:NAD+ oxidoreductase, EC 1.1.1.8) has been isolated and purified from Saccharomyces cerevisiae by affinity and exclusion chromatography. The enzyme was purified 5 1 OO-fold to a specific activity of 158. It has a molecular w