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Expression and purification of human antimicrobial peptide, dermcidin, in Escherichia coli

✍ Scribed by Ingrid Čipáková; Juraj Gašperík; Eva Hostinová

Book ID: 116828180
Publisher: Elsevier Science
Year: 2006
Tongue: English
Weight: 326 KB
Volume: 45
Category: Article
ISSN: 1046-5928
DOI: 10.1016/j.pep.2005.07.002

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A fusion protein was genetically engineered that contains an antimicrobial peptide, designated P2, at its carboxy terminus and bovine prochymosin at its amino terminus. Bovine prochymosin was chosen as the fusion partner because of its complete insolubility in Escherichia coli, a property utilized t