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Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli

✍ Scribed by B. Bommarius; H. Jenssen; M. Elliott; J. Kindrachuk; Mukesh Pasupuleti; H. Gieren; K.-E. Jaeger; R.E.W. Hancock; D. Kalman


Book ID
116832286
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
560 KB
Volume
31
Category
Article
ISSN
0196-9781

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A fusion protein was genetically engineered that contains an antimicrobial peptide, designated P2, at its carboxy terminus and bovine prochymosin at its amino terminus. Bovine prochymosin was chosen as the fusion partner because of its complete insolubility in Escherichia coli, a property utilized t