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Expression and Purification of Full-Length Human Bax α

✍ Scribed by Sylvie Montessuit; Gonzalo Mazzei; Edith Magnenat; Bruno Antonsson


Book ID
115645431
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
88 KB
Volume
15
Category
Article
ISSN
1046-5928

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Expression, purification and spectroscop
✍ Robert N. Leach; Mark R. Boyett; John B.C. Findlay 📂 Article 📅 2003 🏛 Elsevier Science 🌐 English ⚖ 479 KB

A polypeptide corresponding to the full-length C-terminal cytoplasmic domain of a G-protein-regulated inwardly rectifying potassium channel (Kir3.1) bearing a hexahistidine (His6) tag was produced by DNA recombinant overexpression techniques in Escherichia coli. This permitted the isolation of f 5 m