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Expression and Purification of an Active, Full-Length Hepatitis C Viral NS4A

✍ Scribed by Sung H. Back; Jung-Eun Kim; Jungmin Rho; Bumsuk Hahm; Tae-Gyu Lee; Eunice E. Kim; Joong-Myung Cho; Sung K. Jang

Book ID
115645719
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
184 KB
Volume
20
Category
Article
ISSN
1046-5928

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A polypeptide corresponding to the full-length C-terminal cytoplasmic domain of a G-protein-regulated inwardly rectifying potassium channel (Kir3.1) bearing a hexahistidine (His6) tag was produced by DNA recombinant overexpression techniques in Escherichia coli. This permitted the isolation of f 5 m