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Expression and purification of enzymatically active recombinant RNA-dependent RNA polymerase (NS5) of the flavivirus Kunjin

โœ Scribed by Kimberley J Guyatt; Edwin G Westaway; Alexander A Khromykh


Book ID
114139597
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
281 KB
Volume
92
Category
Article
ISSN
0166-0934

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The nonstructural protein 5B (NS5B) of hepatitis C virus (HCV) is considered to possess RNA-dependent RNA polymerase (RdRp) activity and to play an essential role for the viral replication. In this study, we expressed the NS5B protein of 65 kd by a recombinant baculovirus. With the highly purified N