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Expression and Knockdown Analysis of Glucose Phosphate Isomerase in Chicken Primordial Germ Cells

✍ Scribed by Rengaraj, D.; Lee, S. I.; Yoo, M.; Kim, T. H.; Song, G.; Han, J. Y.


Book ID
120253066
Publisher
Society for the Study of Reproduction
Year
2012
Tongue
English
Weight
901 KB
Volume
87
Category
Article
ISSN
0006-3363

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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1Β°C/min until the temperature reached -80Β°C; cells were then