Molecular cloning and expression ofdead endhomologue in chicken primordial germ cells
β Scribed by Shinya Aramaki; Fuminori Sato; Takako Kato; Tomoki Soh; Yukio Kato; Masa-aki Hattori
- Publisher
- Springer-Verlag
- Year
- 2007
- Tongue
- English
- Weight
- 408 KB
- Volume
- 330
- Category
- Article
- ISSN
- 0302-766X
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Gonadal primordial germ cells (gPGCs) were collected from gonadal anlage of 5day-old White Leghorn (WL) embryos. Collected gPGCs were suspended in freezing medium containing 10% dimethyl sulphoxide (DMSO). The cell-suspension was frozen at 1Β°C/min until the temperature reached -80Β°C; cells were then
## Abstract The development of chicken embryonic gonads is locally regulated by the systematic action of growth factors. Recently, we used suppressive subtraction cloning to identify transforming growth factor Ξ²2 (TGFβΞ²2) as a growth factor gene preferentially expressed in chicken embryonic ovaries