✦ LIBER ✦

Expression and function of two nicotinic subunits in insect neurons

✍ Scribed by Vermehren, A. ;Trimmer, B.A.

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 279 KB
Volume: 62
Category: Article
ISSN: 0022-3034
DOI: 10.1002/neu.20088

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✦ Synopsis

Nicotinic acetylcholine receptors (nAChRs) in insects are neuron-specific oligomeric proteins essential for the central transmission of sensory information. Little is known about their subunit composition because it is difficult to express functional insect nAChRs in heterologous systems. As an alternative approach we have examined the native expression of two subunits in neurons of the nicotinic-resistant, tobaccofeeding insect Manduca sexta. Both the ␣-subunit MARA1 and the ␤-subunit MARB can be detected by in situ hybridization in the majority of cultured neurons with an overlapping, but not identical, distribution.

Changes in intracellular Ca 2؉ evoked by nicotinic stimulation are more strongly correlated to the expression of MARA1 than MARB and are independent of cell size. Unlike the previously reported critical role of MARA1 in mediating nicotinic Ca 2؉ responses, down-regulation of MARB by RNA interference (RNAi) did not reduce the number of responding neurons or the size of evoked responses, suggesting that additional subunits remain to be identified in Manduca.

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