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Expression and Characterization of Rat Soluble Catechol-O-methyltransferase Fusion Protein

✍ Scribed by Maria João Bonifácio; Maria Augusta Vieira-Coelho; Patrı́cio Soares-da-Silva


Book ID
115645889
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
94 KB
Volume
23
Category
Article
ISSN
1046-5928

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Purification of rat liver soluble catech
✍ Tapio Korkolainen; Erkki Nissinen 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 324 KB 👁 2 views

The soluble form of catechol-0-methyltransferase (EC 2.1.1.6.) from rat liver was purified to homogeneity by high-performance anion-exchange chromatography and high-performance gel-filtration chromatography. The specific activity of the final pool was 270 U/mg protein. The purification was 1180-fold