To analyze the intracellular catechol-O-methyltransferase (COMT) activity in cultured cells, a substrate (dihydroxybenzoic acid) was added to the tissue culture plates and the product (vanillic acid) was analyzed with reversed-phase HPLC with coulometric detection. The cultures, prepared from variou
β¦ LIBER β¦
Kinetics of rat brain soluble catechol-O-methyltransferase and its inhibition by substrate analogues
β Scribed by Godfrey Tunnicliff; That T. Ngo
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 548 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0020-711X
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The soluble form of catechol-0-methyltransferase (EC 2.1.1.6.) from rat liver was purified to homogeneity by high-performance anion-exchange chromatography and high-performance gel-filtration chromatography. The specific activity of the final pool was 270 U/mg protein. The purification was 1180-fold