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Expression and characterization of Pichia etchellsii β-glucosidase in Escherichia coli

✍ Scribed by Manjula Pandey; Saroj Mishra


Book ID
117522099
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
742 KB
Volume
190
Category
Article
ISSN
0378-1119

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The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible beta-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, comprising ammonium sulfate precipitation, ion-exchange and hydroxyapatite chrom