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Cloning and expression of β-glucosidase gene from the yeast Pichia etchellsii

✍ Scribed by Manjula Pandey; Saroj Mishra


Book ID
113397758
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
962 KB
Volume
80
Category
Article
ISSN
0922-338X

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The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible beta-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, comprising ammonium sulfate precipitation, ion-exchange and hydroxyapatite chrom