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Expression and characterization of human proinsulin fused to thioredoxin inEscherichia coli

✍ Scribed by Trabucchi, Aldana; Guerra, Luciano Lucas; Faccinetti, Natalia Inés; Iacono, Ruben Francisco; Poskus, Edgardo; Valdez, Silvina Noemí

Book ID
113025417
Publisher
Springer
Year
2011
Tongue
English
Weight
402 KB
Volume
94
Category
Article
ISSN
1432-0614

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The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod