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Expressed sequence tag-derived microsatellite markers of perennial ryegrass (Lolium perenneL.)

✍ Scribed by Bruno Studer; Torben Asp; Ursula Frei; Stephan Hentrup; Helena Meally; Aurélie Guillard; Susanne Barth; Hilde Muylle; Isabel Roldán-Ruiz; Philippe Barre; Carole Koning-Boucoiran; Gerda Uenk-Stunnenberg; Oene Dolstra; Leif Skøt; Kirsten P. Skøt; Lesley B. Turner; Mervyn O. Humphreys; Roland Kölliker; Niels Roulund; Klaus K. Nielsen; Thomas Lübberstedt

Book ID
106468979
Publisher
Springer Netherlands
Year
2008
Tongue
English
Weight
237 KB
Volume
21
Category
Article
ISSN
1380-3743

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Stable transformation and long-term expr
Stable transformation and long-term expression of thegusAreporter gene in callus lines of perennial ryegrass (Lolium perenneL.)
✍ Heleen M. Maas; Eliza R. Jong; Saskia Rueb; Lambert A. M. Hensgens; Frans A. Kre 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 543 KB

Stable transformation of perennial ryegrass (Lolium perenne L.) was achieved by biolistic bombardment of a non embryogenic cell suspension culture, using the hpt and gusA gene. The transformation yielded on the average 5 callus lines per bombardment (1.4 x 106 cells). Stable integration of the genes