Stable transformation and long-term expr
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Heleen M. Maas; Eliza R. Jong; Saskia Rueb; Lambert A. M. Hensgens; Frans A. Kre
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Article
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1994
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Springer
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English
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Stable transformation of perennial ryegrass (Lolium perenne L.) was achieved by biolistic bombardment of a non embryogenic cell suspension culture, using the hpt and gusA gene. The transformation yielded on the average 5 callus lines per bombardment (1.4 x 106 cells). Stable integration of the genes