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Expressed Protein Ligation: Method and Applications

✍ Scribed by Ralf David; Michael P. O. Richter; Annette G. Beck-Sickinger


Publisher
John Wiley and Sons
Year
2004
Weight
52 KB
Volume
35
Category
Article
ISSN
0931-7597

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Here we describe the results of studies designed to explore the scope and limitations of expressed protein ligation (EPL), a protein semisynthesis approach that allows unnatural amino acids to be site specifically introduced into large proteins. Using Src homology 3 domains from the proteins c-Abl a