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A Synthetic Kiss of Death: Expressed Protein Ligation of a Ubiquitin–Peptide Conjugate

✍ Scribed by Christian P. R. Hackenberger


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
201 KB
Volume
8
Category
Article
ISSN
1439-4227

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Here we describe the results of studies designed to explore the scope and limitations of expressed protein ligation (EPL), a protein semisynthesis approach that allows unnatural amino acids to be site specifically introduced into large proteins. Using Src homology 3 domains from the proteins c-Abl a