𝔖 Bobbio Scriptorium
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Examination of polysaccharides for their uses in hybridoma cell culture

✍ Scribed by Bing Q. Shen; Steven Reid; Paul F. Greenfield


Book ID
110557956
Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
576 KB
Volume
7
Category
Article
ISSN
0951-208X

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Simplified method of making alginate-pol
✍ Gyun Min Lee; Bernhard O. Palsson πŸ“‚ Article πŸ“… 1990 πŸ› Springer-Verlag 🌐 English βš– 763 KB

The method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPM1 1640 medium. Phosphate concentration in RPM1 1640 medium is sufficiently high to dissolve the alginate gel and, thereby, can be used to eliminate the step of citrat