✦ LIBER ✦
Simplified method of making alginate-polylysine microcapsules for hybridoma cell culture using RPMI 1640 medium
✍ Scribed by Gyun Min Lee; Bernhard O. Palsson
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 763 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0951-208X
No coin nor oath required. For personal study only.
✦ Synopsis
The method of making alginate-poly-L-lysine microcapsules for hybridoma cell culture can be simplified by cultivating the cells in RPM1 1640 medium. Phosphate concentration in RPM1 1640 medium is sufficiently high to dissolve the alginate gel and, thereby, can be used to eliminate the step of citrate buffer treatment required for reliquefying the interior al&ate gel.