Sensitivity of single-strand conformation polymorphism analysis of PCR products (PCR-SSCP analysis) is known to be decreased when the DNA fragments are longer than 300 bp. We examined effects of buffer ions in an attempt to extend the length limit of the analysis. It has been noted that addition of
Exact mass analysis of DNA fragments
โ Scribed by D. Gaudin; K. Jankowski
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 190 KB
- Volume
- 15
- Category
- Article
- ISSN
- 1076-5174
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โฆ Synopsis
Abstract
High resolution data for the major ions from the salmon sperm DNA mass spectrum are reported. The presence of two series of ions for each characteristic fragment is shown, the first containing a phosphate moiety and the second having CHNO compositions only.
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