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Single-Molecule Analysis of Restriction DNA Fragments Using Fluorescence Correlation Spectroscopy

✍ Scribed by Masataka Kinjo; Goro Nishimura; Tomiyasu Koyama; Ülo Mets; Rudolf Rigler


Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
179 KB
Volume
260
Category
Article
ISSN
0003-2697

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Fluorescence correlation spectroscopy (FCS) provides information about translational diffusion properties of fluorescent molecules in tiny detection volume and allows the analysis of binding processes of biomolecules in homogeneous solution. In this study, FCS was used to measure equilibrium binding