We have attempted to optimize the production of stable human cytolytic T lymphocyte clones directed against autologous melanoma cell lines. MLTC were restimulated every week with irradiated melanoma cells in medium containing human serum and IL-2. After 21 to 35 days, in 5 out of 6 patients, these c
Ex vivo generation of human anti-melanoma autologous cytolytic T cells by dendritic cell /melanoma cell hybridomas
β Scribed by Afsaneh Soruri; Afshin Fayyazi; Christine Neumann; Thilo Schlott; Thomas Jung; Constanze Matthes; Joerg Zwirner; Joachim Riggert; J. Hinrich Peters
- Book ID
- 105968464
- Publisher
- Springer-Verlag
- Year
- 2001
- Tongue
- English
- Weight
- 283 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0340-7004
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Limiting numbers of peripheral-blood mononuclear cells (PBMC) from melanoma patients were stimulated with irradiated autologous tumor cells in the presence of interleukins-2 and -4 and in the absence of feeder cells. The responder cells were restimulated every week. After 2 to 4 weeks, the microcult
## We have pursued our analysis of potential tumor-rejection antigens recognized on human melanoma by autologous cytolytic T lymphocytes (CTL). We reported previously that 3 distinct antigens ( A,B,C) were recognized on melanoma cell line SK29-MEL in association with HLA-A2. Selection for melanoma