𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Production of stable cytolytic T-cell clones directed against autologous human melanoma

✍ Scribed by M. Hérin; C. Lemoine; P. Weynants; F. Vessière; A. Van Pel; T. Boon; A. Knuth; R. Devos

Publisher
John Wiley and Sons
Year
1987
Tongue
French
Weight
721 KB
Volume
39
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

We have attempted to optimize the production of stable human cytolytic T lymphocyte clones directed against autologous melanoma cell lines. MLTC were restimulated every week with irradiated melanoma cells in medium containing human serum and IL-2. After 21 to 35 days, in 5 out of 6 patients, these cultures expressed a preferential cytolytic activity against the autologous melanoma cells, as compared to autologous EBV-B cells or N K target K562. Limiting dilution of MLTC responder cells was performed at times varying from days 7 to 28, in medium containing IL-2 and allogeneic EBV-B cells as feeders. Approximately IYo of these responder cells gave rise to CTL clones that lysed the autologous melanoma cells, but did not lyse K562 or autologous B cells. It was possible to maintain in culture for several months a large number of CTL clones that retained this specificity with high activity, and multiplied more than 5-fold every week. Some of these CTL clones were dependent on the presence of the autologous melanoma cells for their growth. With one melanoma, the use of autologous CTL clones made it possible to identify 3 different antigens on the tumor cells.

📜 SIMILAR VOLUMES

Precursor frequency analysis of human cy
Precursor frequency analysis of human cytolytic T lymphocytes directed against autologous melanoma cells
✍ Pierre G. Coulie; Michel Somville; Frédéric Lehmann; Philippe Hainaut; Francis B 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 French ⚖ 893 KB

Limiting numbers of peripheral-blood mononuclear cells (PBMC) from melanoma patients were stimulated with irradiated autologous tumor cells in the presence of interleukins-2 and -4 and in the absence of feeder cells. The responder cells were restimulated every week. After 2 to 4 weeks, the microcult

Multiple sub-sets of Cd4+ and Cd8+ cytot
Multiple sub-sets of Cd4+ and Cd8+ cytotoxic T-cell clones directed to autologous human melanoma identified by cytokine profiles
✍ Cristina Maccalli; Roberta Mortarini; Giorgio Parmiani; Andrea Anichini 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 French ⚖ 861 KB

CD4+ and CD8+ cytotoxic T-cell (CTL) clones, selected for T-cell-receptor (TcR)-dependent lysis of the autologous tumor and isolated from peripheral-blood lymphocytes (PBL) or tumorinfiltrating lymphocytes (TIL) of 3 melanoma patients, were characterized for the pattern of I3 different cytokines rel

Frequent coexpression of cytolytic activ
Frequent coexpression of cytolytic activity and lymphokine production among human T lymphocytes. Production of B cell growth factor and interleukin 2 by T8+ and T4+ cytolytic clones
✍ Maria Cristina Mingari; Alessandro Moretta; Enrico Maggi; Giuseppe Pantaleo; Fra 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 481 KB

## Abstract These studies were designed to determine the degree of overlapping between cytolytic function and lymphokine production among peripheral blood human T lymphocytes. T8^+^ and T4^+^ cells were obtained by sorting purified T cells using the fluorescence‐activated cell sorter (FACS) and clo