Evolution of endogenous hormone concentration in embryogenic cultures of carrot during early expression of somatic embryogenesis
✍ Scribed by V. M. Jiménez; E. Guevara; J. Herrera; F. Bangerth
- Publisher
- Springer
- Year
- 2004
- Tongue
- English
- Weight
- 164 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0721-7714
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The pattern of expression of the rolB gene, derived from the T-DNA of the plant pathogen Agrobacterium rhizogenes, has been investigated during the early stages of somatic embryo formation in suspension cultures of carrot (Daucus carota L.). The reporter gene GUS (Escherichia coli -glucuronidase), u
We report the identification, via their cDNAs, of genes which are temporarily transcribed during the initiation of somatic embryogenesis in carrot (Daucus carota L.) cells cultured in an auxin-free medium. Their expression is roughly associated with the first morphogenetic, or globular, stage. A cDN