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Evidence in support of a role for Ca2+-activated K+ channels in the hamster sperm acrosome reaction

✍ Scribed by Llanos, Miguel N.

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
485 KB
Volume
269
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

The sperm acrosome reaction (AR) is a crucial step for mammalian fertilization. This work describes experiments to test the effect of the cesium ion (Cs^+^) and charybdotoxin (ChTX) on the Ca^2+^ or Na^+^/K^+^ ionophores stimulated hamster sperm AR in vitro. Cs^+^ and ChTX, a polypeptide toxin from the venom of the scorpion Leirus quinquestriatus, are considered blockers of Ca^2+^‐activated K^+^ channels in several somatic cells. Both agents inhibited the AR by 55–66%. The inhibition was completely reversed by the Na^+^/K^+^ ionophore nigericin, but not by the Ca^2+^ ionophore A‐23187. Results give evidence in support of a role for Ca^2+^‐activated K^+^ channels in K^+^ influx required for the occurrence of the hamster sperm acrosome reaction. Β© 1994 Wiley‐Liss, Inc.

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